Isoschizomers and neoschizomers: An isoschizomer is a restriction enzyme that recognizes the (SmaI exhibits 25–50% activity at 37°C.) The recognition sequence and the cutting site usually match, but sometimes the cutting site can be dozens of nucleotides away from the recognition site. Cut Site: CCC GGG GGG CCC. Having supplied restriction enzymes to the research community for over 40 years, NEB has earned the reputation of being the leader in enzyme technologies. 25°C. Time-Saver™ qualified for digestion in 5-15 minutes Isoschizomers enzymes HpaII-MspI and SmaI-XmaI recognize CCGG and CCCGGG, respectively, but HpaII and SmaI lack activity when a methyl group is present in their recognition site [61]. Incubation Conditions: Buffer J. The classical restriction enzymes cut up, and hence render harmless, any unknown (non-cellular) DNA that enters a bacterial cell as a result of a viral infection. Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled quality. Restriction enzymes: Restriction endonucleases are used to enrich methylated from unmethylated DNA. Isoschizomers: TspMI, XmaCI, XmaI. Having supplied restriction enzymes to the research community for over 40 years, NEB has earned the reputation of being the leader in enzyme technologies. Ten enzymes were investigated: seven enzymes with a single cut site (EcoRI, KpnI, NdeI, NotI, NruI, SmaI, XbaI), two enzymes with two cut sites (BstZ17I, EagI), and one enzyme with no cut site (ClaI). Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Source: Serratia marcescens. They recognize a specific DNA sequence, usually short (3 to 8 bp ), and cut it, producing either blunt or overhung ends, either at or nearby the recognition site . The recognition sequence and the cut site usually match, but sometimes the cut site can be dozens of nucleotides away from the recognition site. Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled quality. Most restriction enzymes cut their corresponding restriction sites in a staggered fashion leaving single-stranded overhangs. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. In addition, we observe a decrease in alignment upon further digestion and subsequent shortening of the DNA. Isoschizomers and neoschizomers: An isoschizomer is an enzyme that … Note: XmaI is a neoschizomer of SmaI. 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